为解决传统RT-PCR/RFLP方法对部分日本牛冠状病毒(BCoV)流行株基因分型失效的问题，日本研究人员通过引入AvaII、EcoO65I、AfaI和 ...

The identity of black alder (Alnus glutinosa (L.) Gaertn.) ectomycorrhizas was investigated using PCR/RFLP analysis of the ITS region from 16 morphotypes sampled at a 60-yr-old black alder stand. A ...

为解决当前植物 CRISPR 编辑定量技术差异大、结果可比性不足的问题，研究人员系统评估了 AmpSeq、PCR-RFLP、T7E1、Sanger 测序、PCR ...

We introduce a method of in vitro recombination or “DNA shuffling” to generate libraries of evolved enzymes. The approach relies on the ordering, trimming, and joining of randomly cleaved parental DNA ...

Although most diseases are caused by point mutations, in many cases, genomic duplications or deletions of genes have also been shown to be responsible for the same disorder. Changes in gene copy ...

This is a preview. Log in through your library . Abstract A clinical trial was conducted in Argentina to determine the efficacy of clarithromycin plus lansoprazole for the treatment of Helicobacter ...

Nucleic acid analysis, genotyping, PCR, and RFLP methodologies are time-consuming techniques using agarose or acrylamide gel electrophoresis—image capture plus gel image interpretations. Capillary gel ...

Genetic and proteomic features associated with survival after treatment with erlotinib in first-line therapy of non-small cell lung cancer No significant financial relationships to disclose. This is ...