[摘要]Pyrosequencing是新一代DNA序列分析技术，基于该技术的分析系统结合相应软件和试剂可以进行DNA序列分析，SNP分析和SNP频率确定。本文介绍了Pyrosequencing技术的原理和应用。 [关键词] Pyrosequencing DNA序列分析 SNP分析 DNA序列分析技术是现代生命科学研究的核心 ...

Pyrosequencing是对短到中等长度的DNA序列样品进行高通量的、精确和重复性好的分析的技术。 第一步——测序引物和PCR扩增的、单链的DNA模板杂交，与酶—DNA聚合酶（DNA polymerase）、ATP硫酸化酶（ATP sulfurylase）、荧光素酶（luciferase）、三磷酸腺苷双磷酸酶（apyrase ...

Metatranscriptomes generated by pyrosequencing hold significant potential for describing functional processes in complex microbial communities. Meeting this potential requires protocols that maximize ...

Pyrosequencing can be used to detect DNA and RNA from almost any source, and has applications in bacteria resistance screening, drug development, epigenetics, and many other fields. If no light is ...

Similar to soil and sediment, activated sludge (AS) is a highly complex system of eukaryotes (protozoa and fungi), bacteria, archaea and viruses, in which bacteria are dominant. Despite its importance ...

DNA methylation is a form of epigenetics in which gene expression is encouraged or inhibited by the addition of a methyl group on cytosines. It is highly implicated in development, cell ...

Phase Ib clinical trial of OKN-007 in recurrent malignant glioma. This is an ASCO Meeting Abstract from the 2020 ASCO Annual Meeting I. This abstract does not include a full text component.

The sequencing of DNA molecules started with the development of chemical cleavage method of Maxam-Gilbert during 1970s followed by di-deoxy chain termination method. However, the whole genome ...

Factors influencing outcome in thymic epithelial tumors (TET). This is an ASCO Meeting Abstract from the 2012 ASCO Annual Meeting I. This abstract does not include a full text component.

Roula Khalaf, Editor of the FT, selects her favourite stories in this weekly newsletter. Pal Nyren’s Eureka moment came in 1986 when he was cycling home from Cambridge university. “It is often when ...