Bicoid is one of the important Drosophila maternal genes involved in the control of embryo polarity and larvae segmentation. To clone and characterize the rice bicoid-related genes, one cDNA clone, ...

自20世纪70年代中期首例 cDNA 克隆问世以来，构建 cDNA 文库已成为研究功能基因组学的基本手段之一。cDNA 便于克隆和大量表达，它不像基因组含有内含子而难于表达，因此可以从 cDNA 文库中筛选到所需的目的基因，并直接用于该目的基因的表达。通过构建 cDNA ...

Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with ...

Complementary DNA (cDNA) libraries are standard tools for gene expression studies. In theory, the library contains full-length DNA copies of every mRNA in the starting sample, in abundances ...

Biosynthesis of the anticancer drug Taxol involves 19 enzymatic steps from the universal diterpenoid progenitor geranylgeranyl diphosphate derived by the plastidial methylerythritol phosphate pathway ...

We do cloning from the ORFeome collection, as well as from your own Gateway-compatible PCR products and plasmids to a number of destination vectors. Our selection of vectors includes a wide range of ...

Core laboratories must balance the efficiency of their NGS workflow with the quality of the data that they produce in providing numerous next generation sequencing (NGS) services relating to a wide ...

Figure 1: RNA-seq data uses short reads of mRNA which is free of intronic non-coding DNA. These reads must then be aligned back to the reference genome. Credit: Technology Networks. Early RNA-seq ...

What is an RNA sequencing library? In all RNA-seq experiments, a library of cDNA fragments attached to adapters must be synthesized in order for sequencing to be successful. How do you prepare an RNA ...